Published Studies
Patch testing with 20% dermatophagoides pteronyssimus-farinae antigen
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Patch testing with 20% dermatophagoides pteronyssimus/farinae (chemotechnique) antigen


Jamora J, Verallo-Rowell VM, Samson-Veneracion MT.

PUBLICATION: American Journal of Contact Dermatitis 2001; 12(2): 67-71 
 

Abstract

 
BACKGROUND:
Patch testing with dust mite antigens might identify mite-sensitive individuals, particularly those with atopic dermatitis who can benefit from avoidance measures. Currently available dust mite allergens have not been well studied.
 
OBJECTIVE:
To determine the proper dilution of 20% Dermatophagoides pteronyssinus/farinae mix antigen (Chemotechnique, Malmo, Sweden) for use in closed patch testing.
 
METHODS:
Eighteen nonatopic, healthy control subjects were patch-tested to the 20% concentration, yielding 15 (83%) positive reactions, most showing a decrescendo or persistent pattern suggesting an inordinately high number of false positive reactions. Dilutions of 1.25% to 0.1% in white petrolatum were used in patch testing 8 atopic dermatitis and 11 respiratory atopy patients, and 12 nonatopic controls.
 
RESULTS:
Positive reaction rates to the 0.25% and 0.1% concentrations, respectively, were 87.5% and 62.5% for atopic dermatitis, 54% and 18% for respiratory atopy, and 33% and 8% for healthy controls. Using Fisher's exact test, the 0.1% dilution was shown to significantly differentiate rates of positivity among the 3 groups, particularly between atopic dermatitis subjects and healthy controls.
 
CONCLUSION:
We find that a 0.1% dilution of 20% D. pteronyssinus/farinae mix antigen (Chemotechnique) to be useful in identifying mite-allergic individuals with atopic dermatitis.
 
Copyright 2001 by W.B. Saunders Company.